In-Vitro Organogenesis in Tomato (Solanum Lycopersicum) Using Kinetin

This research was undertaken for optimizing simple and reproducible protocol on indirect regeneration in tomato using kinetin. The effect of kinetin for efficient callus production was studied and its results were interpreted. Hypocotyls and cotyledons were used as explants and effect on callus derived regeneration was greatly influenced by addition of kinetin in media. The individually positioned explants on MS media supplemented with two cytokinins BAP (2.0mg/L) and kinetin (1.75mg/L) proved to produce good embryogenic calli and developed into whole plantlets. Lower and higher kinetin concentrations produce lesser calli and results derived using mean values in this experiment suggest closely equal concentrations of kinetin to other cytokinin can be a potential calli inducer in tomato regeneration. Multiple shoot induction data was recorded and its mean values interpreted. Shoots transferred in shooting elongation media MS+ IAA (0.1mg/L) + Zeatin (2.0mg/L) gave better shooting response (89.4%) and after two weeks these elongated plantlets were subjected to rooting. Therefore kinetin with combination of BAP majorly influences in-vitro organogenesis in tomato plant regeneration.